Device
EXP-CBD_su

Part:BBa_K5007007:Design

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-10-03)


Gal1-Kozak-CBD_sub-ADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 777
    Illegal NgoMIV site found at 2080
    Illegal AgeI site found at 150
    Illegal AgeI site found at 1639
    Illegal AgeI site found at 3820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1320


Design Notes

This device is a transcriptional unit designed specifically for S. cerevisiae. It contains the Gal1 inducible promoter with Kozak sequence and the ADH1 terminator, both quite efficient at starting and terminating the transcription of long ORFs. Under the control of the Gal1 promoter, we can induce the synthesis of olivetolic acid at specific cell densities and growth phases, allowing for optimizations in several steps. Furthermore, part BBa_K5007000 was designed as an interchangeable part, capable of being switched between plasmids and assembled with other promoter-terminator pairs, optimized for different chassis and further elaborated.


Source

Part BBa_J63006 was developed by Silver Lab and further developed and studied by several iGEM teams. The part comes from S. cerevisiae. Part BBa_K392003 was developed by iGEM10 Osaka team, with efficacy shown in different constructs.

References